Super Resolution Microscopy
Ground State Depletion Microscopy
Using MPB 642 & 532-nm Lasers
Mitochondria 647: COS cells stained for ATP-Synthase in inner mitochondrial membrane with Alexa647. Courtesy of Prof. R. Jacob, Philipps University Marburg.
Courtesy ofProf. R. Jacob, Philipps University Marburg
Developments in biological microscopy which result in the nonlinear response of excitation of fluorophores have provided researchers with greater resolution that previously available from standard microscopy techniques. Stimulated Emission Depletion (STED), Ground State Depletion (GSD), Saturated Structured Illumination (SSIM), Stochastic optical reconstruction microscopy (STORM), photo activated localization microscopy (PALM) and fluorescence photo-activation localization microscopy (fPALM) are among the techniques used in Super Resolution Microscopy to have benefitted from the emergence of high power laser systems.
MPB Communications is pleased to have developed High Power Visible Fiber Lasers in partnership with leading research laboratories worldwide which target wavelengths previously unavailable. The flexibility of our technology allows for the development of novel emission wavelengths for use in Super Resolution Microscopy where beam quality (TEM00, M2 < 1.1) and stability is a must (see Why M2 is Important). Using these novel wavelengths our research partners have been able to open up areas of investigation which have led to ground breaking developments.
MPBC is proud to have been a small part of this new era and looks forward to the continued success of its partners.
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For more information on laser selection, see “Why M2 is Important When Selecting Your Laser.” |
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Download our "Visible Fiber Lasers for Bioscience" brochure |
STED Imaging - Using MPB 775-nm Frequency-Doubled Pulsed Fiber Laser
Courtesy Max Plank Institute
Three-color STED nanoscopy with one STED wavelength and excitation multiplexing. ATTO 590, ATTO 647N and KK114 were combined in one cell staining.
(A) Unmixed RGB image of vimentin-ATTO 590 (red), α-actinin-KK114 (green) and lamin-ATTO 647N (blue) in STED and confocal microcopy images.
(B-D) raw STED microscopy images of (A). (A) was smoothed with a Wiener filter.
Görlitz, F., P. Hoyer, H. J. Falk, L. Kastrup, J. Engelhardt, S. W. Hell: "A STED Microscope Designed for Routine Biomedical Applications" Progress in Electromatetics Research, Vol.147, 57 - 68 (2014).
See how some of our customers are using our lasers
Microscopy
- Alan M. Szalai, Lucía F. Lopez, Miguel Ángel Morales-Vásquez, Fernando D. Stefani and Pedro F. Aramendí, "Analysis of sparse molecular distributions in fibrous arrangements based on the distance to the first neighbor in single molecule localization microscopy." Nanoscale, Issue 17, 2020.
- Ströhl F., Lin J.Q., van Tartwijk F.W., Wong H.HW., Holt C.E., Kaminski C.F. (2020). "A Protocol for Single-Molecule Translation Imaging in Xenopus Retinal Ganglion Cells." In: Yamamoto N., Okada Y. (eds) Single Molecule Microscopy in Neurobiology. Neuromethods, vol 154. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0532-5_14.
- Köbele, L., Rohrbach, A. "A shape-switch-block method for confocal light-sheet microscopy with sectioned Bessel beams and stimulated emission depletion." Communications Physics, 2020.https://doi.org/10.1038/s42005-020-00458-3.
- Morozumi, A., Kamiya, M., Urano, Y., "Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties." Single Molecule Microscopy in Neurobiology (pp.203-227).
- Felix Wäldchen, F., Schlegel, J., Götz, R., Luciano, M., Schnermann, M., Doose, S. & Sauer, M. "Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM." Nature Communications, volume 11, Article number: 887 (2020).
- Zhang, Y., Schroeder, L.K., Lessard, M.D. et al. "Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging." Nature Methods 17, 225–231 (2020). https://doi.org/10.1038/s41592-019-0676-4
- Wensheng Wang, Zhimin Zhang, Shancong Liu, Yuchen Chen, Liang Xu, Cuifang Kuang, Xiang Hao, Xu Liu, "Stimulated emission depletion microscopy with array detection and photon reassignment," Optics and Lasers in Engineering, Volume 129, 2020, 106061,https://doi.org/10.1016/j.optlaseng.2020.106061.
Structured Illumination Microscopy
- Elbaz-Alon, Y., Guo, Y., Segev, N. et al. "PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria." Nature Communications 11, 3645 (2020). https://doi.org/10.1038/s41467-020-17451-7.
Lattice Light-Sheet
- Bin Cao, Guanshi Wang, Jieru Li, Alexandros Pertsinidis, "3D Interferometric Lattice Light-Sheet Imaging," doi: https://doi.org/10.1101/2020.08.27.266999